Determination of anti-ds-DNA antibodies by three different methods: Comparison of sensitivity, specificity and correlation with lupus activity index (LAI)
Identifieur interne : 003079 ( Main/Exploration ); précédent : 003078; suivant : 003080Determination of anti-ds-DNA antibodies by three different methods: Comparison of sensitivity, specificity and correlation with lupus activity index (LAI)
Auteurs : A. G. Tzioufas [Grèce] ; C. Terzoglou [Grèce] ; E. D. Stavropoulos [Grèce] ; S. Athanasiadou [Grèce] ; H. M. Moutsopoulos [Grèce]Source :
- Clinical Rheumatology [ 0770-3198 ] ; 1990-06-01.
English descriptors
- KwdEn :
- Teeft :
- Arthritis rheum, Assay, Avidity, Avidity antibodies, Clinical activity, Clinical immunology laboratory, Crithidia, Crithidia assay, Crithidia assays, Crithidia lucilliae, Crithidia lucilliae assay, Diagnostic criteria, Different methods, Disease activity, Elisa, Elisa values, Enzyme immunoassay, Erythematosus, Farr, Farr assay, Farr assay values, High activity, High affinity antibodies, High avidity, Immunol, Lucilliae, Lupus, Lupus activity, Lupus activity index, Moderate activity, Optical density, Previous reports, Rheum, Rheumatoid arthritis, Room temperature, Sensitive method, Serum levels, Serum samples, Systemic lupus erythematosus.
Abstract
Summary: Ninety-seven sera, 58 from patients with SLE and 39 from patients with other autoimmune rheumatic diseases were tested for anti-ds-DNA antibody activity by ELISA, Farr, and Crithidia Lucilliae assays. Fifty-six per cent of the sera were positive by at least one method. Eighty per cent of the SLE population was positive by ELISA, 39% by Farr Assay and 33% by the Crithidia assay. Crithidia assay exhibited the greater specificity (100%) followed by the Farr Assay (97%) and the ELISA (80%). Sera positive by all methods showed a significantly higher mean value of the ELISA rates, than sera positive only by ELISA (p<0.001). When the SLE sera were analyzed according to disease activity, it was shown that ELISA and Farr Assay correlated well with the lupus activity index (LAI) (r<0.001). The SLE sera with the higher anti-ds-DNA concentration (sera positive by all 3 methods) did not correlate with LAI when tested by the Farr Assay (0.05
Url:
DOI: 10.1007/BF02031967
Url:
DOI: 10.1007/BF02031967
Affiliations:
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Fifty-six per cent of the sera were positive by at least one method. Eighty per cent of the SLE population was positive by ELISA, 39% by Farr Assay and 33% by the Crithidia assay. Crithidia assay exhibited the greater specificity (100%) followed by the Farr Assay (97%) and the ELISA (80%). Sera positive by all methods showed a significantly higher mean value of the ELISA rates, than sera positive only by ELISA (p<0.001). When the SLE sera were analyzed according to disease activity, it was shown that ELISA and Farr Assay correlated well with the lupus activity index (LAI) (r<0.001). The SLE sera with the higher anti-ds-DNA concentration (sera positive by all 3 methods) did not correlate with LAI when tested by the Farr Assay (0.05</div> </front></TEI><affiliations><list><country><li>Grèce</li></country><region><li>Attique (région)</li></region><settlement><li>Athènes</li></settlement></list><tree><country name="Grèce"><noRegion><name sortKey="Tzioufas, A G" sort="Tzioufas, A G" uniqKey="Tzioufas A" first="A. G." last="Tzioufas">A. G. Tzioufas</name></noRegion><name sortKey="Athanasiadou, S" sort="Athanasiadou, S" uniqKey="Athanasiadou S" first="S." last="Athanasiadou">S. Athanasiadou</name><name sortKey="Moutsopoulos, H M" sort="Moutsopoulos, H M" uniqKey="Moutsopoulos H" first="H. M." last="Moutsopoulos">H. M. Moutsopoulos</name><name sortKey="Stavropoulos, E D" sort="Stavropoulos, E D" uniqKey="Stavropoulos E" first="E. D." last="Stavropoulos">E. D. Stavropoulos</name><name sortKey="Terzoglou, C" sort="Terzoglou, C" uniqKey="Terzoglou C" first="C." last="Terzoglou">C. Terzoglou</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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